Method Development and Validation for the Analysis of Ciprofloxacin in Bulk and Local Tablet Formulations using Buffer Mediated Reverse Phase HPLC

Authors

  • Attaullah Bukhari Department of Chemistry, The University of Lahore, Lahore 54590, Pakistan
  • Israr Ahmed Department of Chemistry, The University of Lahore, Lahore 54590, Pakistan

Abstract

A facile and accurate buffer mediated reverse phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for the determination of ciprofloxacin in bulk and local marketed tablet formulations. The developed method was validated as per United State Pharmacopeia (U.S.P) guidelines. This method was validated by looking into consideration the linearity, range, accuracy, precision, limit of quantitation (LOQ), limit of detection (LOD), selectivity and robustness and ruggedness. Marketed tablets of ciprofloxacin and bulk were analyzed in the developed analytical method. Linearity of ciprofloxacin was observed in the calibration curve ranges from 1 to 5 µg/mL and sharp peak was observed at a retention time of 6.244 min. The LOD and LOQ were recorded as 0.0050 and 0.0152 µg/mL respectively. Sensitivity of this method is much better than HPLC method reported 0.11, 0.35 μg/mL. This work remarkably demonstrated 99.55% repeatability, 99.49% and 99.65% intraday and inter day precision, 99.27% accuracy. This was a real improvement for the detection of ciprofloxacin using buffer via the rapid, accurate, precise, and sensitive RP-HPLC method. The pharmaceutical companies can use this method for the qualitative and quantitative determination of Ciprofloxacin in bulk and local tablet formulations.

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Published

2024-06-10

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Section

Research Articles